Mutate everything - mapping the energetic and allosteric landscapes of proteins at scale - Prof. Ben Lehner

We are pleased to confirm that Prof. Ben Lehner will be presenting on Wednesday, March 25th 2026.

Zoom meeting: https://ed-ac-uk.zoom.us/j/88965561204

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Title: Mutate everything: mapping the energetic and allosteric landscapes of proteins at scale

Abstract:

The objective of the new Generative and Synthetic Genomics program at the Wellcome Sanger Institute is to combine data production at scale and AI/machine learning to accelerate our ability to understand, predict and engineer biological systems. Towards this goal we have developed methods that combine mutagenesis with model fitting to quantify the effects of millions of sequence variants on the properties of proteins and RNAs, including protein stability, aggregation, binding affinities, allostery, and expression. This has included site saturation mutagenesis of >1000 human protein domains and >500 alternatively spliced exons, using mutations to probe the structures of amyloid nucleation transitions states, and generating the first complete experimental allosteric maps of proteins, including protein interaction domains, enzymes, receptors, and transcription factors.